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DHX40 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DHX40 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DHX40 antibodies →
$504.00each
Catalog Number: LS-G109892-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DHX40 (NM_024612), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DHX40 | DDX40 | PAD | Protein PAD | ARG147 | DEAH box protein 40
Origin Species
Human
NCBI GenBank
NM_024612

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSRFPAVAGR APRRQEEGER SRDLQEERLS AVCIADREEK GCTSQEGGTT PTFPIQKQRK
61KIIQAVRDNS FLIVTGNTGS GKTTQLPKYL YEAGFSQHGM IGVTQPRKVA AISVAQRVAE
121EMKCTLGSKV GYQVRFDDCS SKETAIKYMT DGCLLKHILG DPNLTKFSVI ILDEAHERTL
181TTDILFGLLK KLFQEKSPNR KEHLKVVVMS ATMELAKLSA FFGNCPIFDI PGRLYPVREK
241FCNLIGPRDR ENTAYIQAIV KVTMDIHLNE MAGDILVFLT GQFEIEKSCE LLFQMAESVD
301YDYDVQDTTL DGLLILPCYG SMTTDQQRRI FLPPPPGIRK CVISTNISAT SLTIDGIRYV
361VDGGFVKQLN HNPRLGLDIL EVVPISKSEA LQRSGRAGRT SSGKCFRIYS KDFWNQCMPD
421HVIPEIKRTS LTSVVLTLKC LAIHDVIRFP YLDPPNERLI LEALKQLYQC DAIDRSGHVT
481RLGLSMVEFP LPPHLTCAVI KAASLDCEDL LLPIAAMLSV ENVFIRPVDP EYQKEAEQRH
541RELAAKAGGF NDFATLAVIF EQCKSSGAPA SWCQKHWIHW RCLFSAFRVE AQLRELIRKL
601KQQSDFPKET FEGPKHEVLR RCLCAGYFKN VARRSVGRTF CTMDGRGSPV HIHPSSALHE
661QETKLEWIIF HEVLVTTKVY ARIVCPIRYE WVRDLLPKLH EFNAHDLSSV ARREVREDAR
721RRWTNKENVK QLKDGISKDV LKKMQRRNDD KSISDARARF LERKQQRTQD HSDTRKETG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

779 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.