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DHX34 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DHX34 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DHX34 antibodies →
$725.00each
Catalog Number: LS-G113781-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DHX34 (NM_014681), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DDX34 | DEAH box protein 34 | DHX34 | KIAA0134
Origin Species
Human
NCBI GenBank
NM_014681
SwissProt
Q14147

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
127.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPPPRTREGR DRRDHHRAPS EEEALEKWDW NCPETRRLLE DAFFREEDYI RQGSEECQKF
61WTFFERLQRF QNLKTSRKEE KDPGQPKHSI PALADLPRTY DPRYRINLSV LGPATRGSQG
121LGRHLPAERV AEFRRALLHY LDFGQKQAFG RLAKLQRERA ALPIAQYGNR ILQTLKEHQV
181VVVAGDTGCG KSTQVPQYLL AAGFSHVACT QPRRIACISL AKRVGFESLS QYGSQVGYQI
241RFESTRSAAT KIVFLTVGLL LRQIQREPSL PQYEVLIVDE VHERHLHNDF LLGVLQRLLP
301TRPDLKVILM SATINISLFS SYFSNAPVVQ VPGRLFPITV VYQPQEAEPT TSKSEKLDPR
361PFLRVLESID HKYPPEERGD LLVFLSGMAE ISAVLEAAQT YASHTQRWVV LPLHSALSVA
421DQDKVFDVAP PGVRKCILST NIAETSVTID GIRFVVDSGK VKEMSYDPQA KLQRLQEFWI
481SQASAEQRKG RAGRTGPGVC FRLYAESDYD AFAPYPVPEI RRVALDSLVL QMKSMSVGDP
541RTFPFIEPPP PASLETAILY LRDQGALDSS EALTPIGSLL AQLPVDVVIG KMLILGSMFS
601LVEPVLTIAA ALSVQSPFTR SAQSSPECAA ARRPLESDQG DPFTLFNVFN AWVQVKSERS
661RNSRKWCRRR GIEEHRLYEM ANLRRQFKEL LEDHGLLAGA QAAQVGDSYS RLQQRRERRA
721LHQLKRQHEE GAGRRRKVLR LQEEQDGGSS DEDRAGPAPP GASDGVDIQD VKFKLRHDLA
781QLQAAASSAQ DLSREQLALL KLVLGRGLYP QLAVPDAFNS SRKDSDQIFH TQAKQGAVLH
841PTCVFAGSPE VLHAQELEAS NCDGSRDDKD KMSSKHQLLS FVSLLETNKP YLVNCVRIPA
901LQSLLLFSRS LDTNGDCSRL VADGWLELQL ADSESAIRLL AASLRLRARW ESALDRQLAH
961QAQQQLEEEE EDTPVSPKEV ATLSKELLQF TASKIPYSLR RLTGLEVQNM YVGPQTIPAT
1021PHLPGLFGSS TLSPHPTKGG YAVTDFLTYN CLTNDTDLYS DCLRTFWTCP HCGLHAPLTP
1081LERIAHENTC PQAPQDGPPG AEEAALETLQ KTSVLQRPYH CEACGKDFLF TPTEVLRHRK
1141QHV
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1143 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.