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DHX32 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DHX32 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DHX32 antibodies →
$504.00each
Catalog Number: LS-G111901-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DHX32 (NM_018180), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DDX32 | DHLP1 | DEAD/H helicase-like protein-1 | HuDDX32 | DEAD/H box 32 | DEAD/H helicase-like protein 1 | DEAH box protein 32 | DHX32
Origin Species
Human
NCBI GenBank
NM_018180
SwissProt
Q7L7V1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEEGLECPN SSSEKRYFPE SLDSSDGDEE EVLACEDLEL NPFDGLPYSS RYYKLLKERE
61DLPIWKEKYS FMENLLQNQI VIVSGDAKCG KSAQVPQWCA EYCLSIHYQH GGVICTQVHK
121QTVVQLALRV ADEMDVNIGH EVGYVIPFEN CCTNETILRY CTDDMLQREM MSNPFLGSYG
181VIILDDIHER SIATDVLLGL LKDVLLARPE LKLIINSSPH LISKLNSYYG NVPVIEVKNK
241HPVEVVYLSE AQKDSFESIL RLIFEIHHSG EKGDIVVFLA CEQDIEKVCE TVYQGSNLNP
301DLGELVVVPL YPKEKCSLFK PLDETEKRCQ VYQRRVVLTT SSGEFLIWSN SVRFVIDVGV
361ERRKVYNPRI RANSLVMQPI SQSQAEIRKQ ILGSSSSGKF FCLYTEEFAS KDMTPLKPAE
421MQEANLTSMV LFMKRIDIAG LGHCDFMNRP APESLMQALE DLDYLAALDN DGNLSEFGII
481MSEFPLDPQL SKSILASCEF DCVDEVLTIA AMVTAPNCFS HVPHGAEEAA LTCWKTFLHP
541EGDHFTLISI YKAYQDTTLN SSSEYCVEKW CRDYFLNCSA LRMADVIRAE LLEIIKRIEL
601PYAEPAFGSK ENTLNIKKAL LSGYFMQIAR DVDGSGNYLM LTHKQVAQLH PLSGYSITKK
661MPEWVLFHKF SISENNYIRI TSEISPELFM QLVPQYYFSN LPPSESKDIL QQVVDHLSPV
721STMNKEQQMC ETCPETEQRC TLQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

743 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.