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DGKG Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DGKG Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DGKG antibodies →
$725.00each
Catalog Number: LS-G119705-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DGKG (NM_001080745), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DAG kinase gamma | Diacylglycerol kinase gamma | DGKG | Diacylglyerol kinase gamma | Diglyceride kinase gamma | DGK-GAMMA | DAGK3
Origin Species
Human
NCBI GenBank
NM_001346
SwissProt
P49619

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGEERWVSLT PEEFDQLQKY SEYSSKKIKD ALTEFNEGGS LKQYDPHEPI SYDVFKLFMR
61AYLEVDLPQP LSTHLFLAFS QKPRHETSDH PTEGASNSEA NSADTNIQNA DNATKADEAC
121APDTESNMAE KQAPAEDQVA ASPLEPPVPR SSSSESPVVY LKDVVCYLSL LETGRPQDKL
181EFMFRLYDSD ENGLLDQAEM DCIVNQMLHI AQYLEWDPTE LRPILKEMLQ GMDYDRDGFV
241SLQEWVHGGM TTIPLLVLLG MDDSGSKGDG RHAWTMKHFK KPTYCNFCHI MLMGVRKQGL
301CCTYCKYTVH ERCVSKNIPG CVKTYSKAKR SGEFHRKCEL STLCDGGELR DHILLPTSIC
361PITRDRPGEK SDGCVSAKGE LVMQYKIIPT PGTHPLLVLV NPKSGGRQGE RILRKFHYLL
421NPKQVFNLDN GGPTPGLNFF RDTPDFRVLA CGGDGTVGWI LDCIDKANFA KHPPVAVLPL
481GTGNDLARCL RWGGGYEGGS LTKILKDIEQ SPLVMLDRWH LEVIPREEVE NGDQVPYSIM
541NNYFSIGVDA SIAHRFHVMR EKHPEKFNSR MKNKLWYFEF GTSETFAATC KKLHDHIELE
601CDGVGVDLSN IFLEGIAILN IPSMYGGTNL WGENKKNRAV IRESRKGVTD PKELKFCVQD
661LSDQLLEVVG LEGAMEMGQI YTGLKSAGRR LAQCASVTIR TNKLLPMQVD GEPWMQPCCT
721IKITHKNQAP MMMGPPQKSS FFSLRRKSRS KD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

752 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.