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DGKD Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DGKD Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DGKD antibodies →
$725.00each
Catalog Number: LS-G105977-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DGKD (NM_152879), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
130 kDa diacylglycerol kinase | DGK-delta | DGKD | Dgkd-2 | DGKdelta | DAG kinase delta | Diacylglycerol kinase delta | Diacylglycerol kinase eta | Diglyceride kinase delta | KIAA0145
Origin Species
Human
NCBI GenBank
NM_003648
SwissProt
Q16760

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
134.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAAGAPPP GPPQPPPPPP PEESSDSEPE AEPGSPQKLI RKVSTSGQIR QKTIIKEGML
61TKQNNSFQRS KRRYFKLRGR TLYYAKTAKS IIFDEVDLTD ASVAESSTKN VNNSFTVITP
121CRKLILCADN RKEMEDWIAA LKTVQNREHF EPTQYSMDHF SGMHNWYACS HARPTYCNVC
181REALSGVTSH GLSCEVCKFK AHKRCAVRAT NNCKWTTLAS IGKDIIEDAD GIAMPHQWLE
241GNLPVSAKCT VCDKTCGSVL RLQDWRCLWC KAMVHTSCKE SLLTKCPLGL CKVSVIPPTA
301LNSIDSDGFW KASCPPSCTS PLLVFVNSKS GDNQGVKFLR RFKQLLNPAQ VFDLMNGGPH
361LGLRLFQKFD TFRILVCGGD GSVGWVLSEI DSLNLHKQCQ LGVLPLGTGN DLARVLGWGS
421ACDDDTQLPQ ILEKLERAST KMLDRWSVMA YEAKLPRQAS SSTVTEDFSE DSEVQQILFY
481EDSVAAHLSK ILTSDQHSVV ISSAKVLCET VKDFVARVGK AYEKTTESSE ESEVMAKKCS
541VLKEKLDSLL KTLDDESQAS SSLPNPPPTI AEEAEDGDGS GSICGSTGDR LVASACPARP
601QIFRPREQLM LRANSLKKAI RQIIEHTEKA VDEQNAQTQE QEGFVLGLSE SEEKMDHRVC
661PPLSHSESFG VPKGRSQRKV SKSPCEKLIS KGSLSLGSSA SLPPQPGSRD GLPALNTKIL
721YPNVRAGMSG SLPGGSVISR LLINADPFNS EPETLEYYTE KCVMNNYFGI GLDAKISLDF
781NNKRDEHPEK CRSRTKNMMW YGVLGTKELL HRTYKNLEQK VLLECDGRPI PLPSLQGIAV
841LNIPSYAGGT NFWGGTKEDD TFAAPSFDDK ILEVVAVFGS MQMAVSRVIR LQHHRIAQCR
901TVKISILGDE GVPVQVDGEA WVQPPGYIRI VHKNRAQTLT RDRAFESTLK SWEDKQKCEL
961PRPPSCSLHP EMLSEEEATQ MDQFGQAAGV LIHSIREIAQ SHRDMEQELA HAVNASSKSM
1021DRVYGKPRTT EGLNCSFVLE MVNNFRALRS ETELLLSGKM ALQLDPPQKE QLGSALAEMD
1081RQLRRLADTP WLCQSAEPGD EESVMLDLAK RSRSGKFRLV TKFKKEKNNK NKEAHSSLGA
1141PVHLWGTEEV AAWLEHLSLC EYKDIFTRHD IRGSELLHLE RRDLKDLGVT KVGHMKRILC
1201GIKELSRSAP AVEA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1214 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.