Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DGKB / DGK Beta Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116880-100
Catalog Number: LS-G116880
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DGKB (NM_004080), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 90 kDa diacylglycerol kinase | DGK | DGKB | Diglyceride kinase beta | DAGK2 | DAG kinase beta | DGK-BETA | Diacylglycerol kinase beta | KIAA0718
- Origin Species
- Human
- NCBI GenBank
- AX032742
- SwissProt
- Q9Y6T7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 90.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK QDILNQTIDF61EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPMVKS KPALLSGGLR MNKGAITPPR121TTSPANTCSP EVIHLKDIVC YLSLLERGRP EDKLEFMFRL YDTDGNGFLD SSELENIISQ181MMHVAEYLEW DVTELNPILH EMMEEIDYDH DGTVSLEEWI QGGMTTIPLL VLLGLENNVK241DDGQHVWRLK HFNKPAYCNL CLNMLIGVGK QGLCCSFCKY TVHERCVARA PPSCIKTYVK301SKRNTDVMHH YWVEGNCPTK CDKCHKTVKC YQGLTGLHCV WCQITLHNKC ASHLKPECDC361GPLKDHILPP TTICPVVLQT LPTSGVSVPE ERQSTVKKEK SGSQQPNKVI DKNKMQRANS421VTVDGQGLQV TPVPGTHPLL VFVNPKSGGK QGERIYRKFQ YLLNPRQVYS LSGNGPMPGL481NFFRDVPDFR VLACGGDGTV GWVLDCIEKA NVGKHPPVAI LPLGTGNDLA RCLRWGGGYE541GENLMKILKD IENSTEIMLD RWKFEVIPND KDEKGDPVPY SIINNYFSIG VDASIAHRFH601IMREKHPEKF NSRMKNKFWY FEFGTSETFS ATCKKLHESV EIECDGVQID LINISLEGIA661ILNIPSMHGG SNLWGESKKR RSHRRIEKKG SDKRTTVTDA KELKFASQDL SDQLLEVVGL721EGAMEMGQIY TGLKSAGRRL AQCSCVVIRT SKSLPMQIDG EPWMQTPCTI KITHKNQAPM781LMGPPPKTGL FCSLVKRTRN RSKETRTRPLEQKLISEEDLAANDILDYKDDDDKV
804 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.