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DEPDC7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DEPDC7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DEPDC7 antibodies →
$504.00each
Catalog Number: LS-G119962-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DEPDC7 (NM_001077242), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DEP domain containing 7 | DJ85M6.4 | DEPDC7 | Novel 58.3 KDA protein | TR2 | Protein TR2/D15
Origin Species
Human
NCBI GenBank
C06131
SwissProt
Q96QD5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATVQEKAAA LNLSALHSPA HRPPGFSVAQ KPFGATYVWS SIINTLQTQV EVKKRRHRLK
61RHNDCFVGSE AVDVIFSHLI QNKYFGDVDI PRAKVVRVCQ ALMDYKVFEA VPTKVFGKDK
121KPTFEDSSCS LYRFTTIPNQ DSQLGKENKL YSPARYADAL FKSSDIRSAS LEDLWENLSL
181KPANSPHVNI SATLSPQVIN EVWQEETIGR LLQLVDLPLL DSLLKQQEAV PKIPQPKRQS
241TMVNSSNYLD RGILKAYSDS QEDEWLSAAI DCLEYLPDQM VVEISRSFPE QPDRTDLVKE
301LLFDAIGRYY SSREPLLNHL SDVHNGIAEL LVNGKTEIAL EATQLLLKLL DFQNREEFRR
361LLYFMAVAAN PSEFKLQKES DNRMVVKRIF SKAIVDNKNL SKGKTDLLVL FLMDHQKDVF
421KIPGTLHKIV SVKLMAIQNG RDPNRDAGYI YCQRIDQRDY SNNIEKTTKD ELLNLLKTLD
481EDSKLSAKEK KKLLGQFYKC HPDIFIEHFG D
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

511 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.