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DEPDC1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DEPDC1B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DEPDC1B antibodies →
$504.00each
Catalog Number: LS-G126582-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DEPDC1B (NM_001145208), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Breast cancer cell 3 | DEPDC1B | DEP domain containing 1B | HBxAg transactivated protein 1 | XTP1 | XTP8
Origin Species
Human
NCBI GenBank
NM_001145208
SwissProt
Q8WUY9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEHRIVGPGP YRATRLWNET VELFRAKMPL RKHRCRFKSY EHCFTAAEAV DWLHELLRCS
61QNFGPEVTRK QTVQLLKKFL KNHVIEDIKG KWGEEDFEDN RHLYRFPPSS PLKPYPKKPP
121NQKDVIKFPE WNDLPPGTSQ ENIPVRPVVM NSEMWYKRHS IAIGEVPACR LVHRRQLTEA
181NVEEIWKSMT LSYLQKILGL DSLEEVLDVK LVNSKFIIHN VYSVSKQGVV ILDDKSKELP
241HWVLSAMKCL ANWPNCSDLK QPMYLGFEKD VFKTIADYYG HLKEPLLTFH LFDAFVSVLG
301LLQKEKVAVE AFQICCLLLP PENRRKLQLL MRMMARICLN KEMPPLCDGF GTRTLMVQTF
361SRCILCSKDE VDLDELLAAR LVTFLMDNYQ EILKVPLALQ TSIEERVAHL RRVQFQKSYP
421EVYQERFPTP ESAALLFPEK PKPKPQLLMW ALKKPFQPFQ RTRSFRM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

467 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.