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DEPDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DEPDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DEPDC1 antibodies →
$504.00each
Catalog Number: LS-G112228-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DEPDC1 (NM_017779), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DEP domain containing 1 | DEPDC1 | DEPDC1A | DEP.8 | SDP35 | DEPDC1-V2
Origin Species
Human
NCBI GenBank
NM_017779
SwissProt
Q5TB30

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESQGVPPGP YRATKLWNEV TTSFRAGMPL RKHRQHFKKY GNCFTAGEAV DWLYDLLRNN
61SNFGPEVTRQ QTIQLLRKFL KNHVIEDIKG RWGSENVDDN NQLFRFPATS PLKTLPRRYP
121ELRKNNIENF SKDKDSIFKL RNLSRRTPKR HGLHLSQENG EKIKHEIINE DQENAIDNRE
181LSQEDVEEVW RYVILIYLQT ILGVPSLEEV INPKQVIPQY IMYNMANTSK RGVVILQNKS
241DDLPHWVLSA MKCLANWPRS NDMNNPTYVG FERDVFRTIA DYFLDLPEPL LTFEYYELFV
301NILGLLQPHL ERVAIDALQL CCLLLPPPNR RKLQLLMRMI SRMSQNVDMP KLHDAMGTRS
361LMIHTFSRCV LCCAEEVDLD ELLAGRLVSF LMDHHQEILQ VPSYLQTAVE KHLDYLKKGH
421IENPGDGLFA PLPTYSYCKQ ISAQEFDEQK VSTSQAAIAE LLENIIKNRS LPLKEKRKKL
481KQFQKEYPLI YQKRFPTTES EAALFGDKPT IKQPMLILRK PKFRSLR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

527 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.