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DENND6B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DENND6B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DENND6B antibodies →
$504.00each
Catalog Number: LS-G122717-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM116B(DENND6B) (NM_001001794), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AFI1B | DENND6B | DENN/MADD domain containing 6B | FAM116B | Protein FAM116B
Origin Species
Human
NCBI GenBank
NM_001001794
SwissProt
Q8NEG7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDALLGTGPR RARGCLGAAG PTSSGRAART PAAPWARFSA WLECVCVVTF DLELGQALEL
61VYPNDFRLTD KEKSSICYLS FPDSHSGCLG DTQFSFRMRQ CGGQRSPWHA DDRHYNSRAP
121VALQREPAHY FGYVYFRQVK DSSVKRGYFQ KSLVLVSRLP FVRLFQALLS LIAPEYFDKL
181APCLEAVCSE IDQWPAPAPG QTLNLPVMGV VVQVRIPSRV DKSESSPPKQ FDQENLLPAP
241VVLASVHELD LFRCFRPVLT HMQTLWELML LGEPLLVLAP SPDVSSEMVL ALTSCLQPLR
301FCCDFRPYFT IHDSEFKEFT TRTQAPPNVV LGVTNPFFIK TLQHWPHILR VGEPKMSGDL
361PKQVKLKKPS RLKTLDTKPG LYTAYTAHLH RDKALLKRLL KGVQKKRPSD VQSALLRRHL
421LELTQSFIIP LEHYMASLMP LQKSITPWKT PPQIQPFSQD DFLRSLEHAG PQLTCILKGD
481WLGLYRRFFK SPHFDGWYRQ RHKEMALKLE ALHLEAICEA NIETWMKDKS EVEVVDLVLK
541LREKLVRAQG HQLPVKEATL QRAQLYIETV IGSLPKDLQA VLCPP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

585 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.