LSBio
DENND2C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DENND2C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DENND2C antibodies →
$725.00each
Catalog Number: LS-G103707-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DENND2C (NM_198459), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DENN/MADD domain containing 2C | DENND2C | DJ1156J9.1 | RP5-1156J9.1
Origin Species
Human
SwissProt
Q68D51

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDVGFSRTTV QTLSRSHCKN IKQKISQWEG RANGISNPEK WCPKDFGVRY NCHQEIRLKK
61NPIAERKSKN LDVTSRENVG LDINENTKSH DQSENENKKH EYDDTHFFKN ESESNWVCSR
121VKEIESCKED VLDPETSLPP GNFYTSQILW KKIEALPPDK LLNLALEHCD SSEKELNFRV
181LDSSYGITKS LENIYSEPEG QECGPSINPL PKPRRTFRYL SESGVTPYKE RNCDKKYCEN
241NSCAQSSLAS SQEPEPKKYG GKIRGRSKRK SFEFEDIQHF RNRNSQTIRE ELGRNSGSAL
301YYTQSEDNIY EDIIYPTKEN PYEDIPVQPL PMWRSPSAWK LPPAKSAFKA PKLVLKIDDI
361FESKRGKKKV KLHSYTGKEL PPTKGETSGN ESDAEYLPKN RHKRLAQLQP SSKRNPHYQT
421LERDLIELQE QQLFELFVVV SLQKKPSGIS YIPQVIQQFP GKDDHGYKQS KDMEERLKVI
481PKFCFPDSKD WMPTSELKSE TFSFVLTGED GSRWFGYCKK LLPVGKGKRL PEVYCMVSRL
541GCFNLFSKIL DEVEKRREMS PALVYPFMRS VMEAPFPAPG RTITVKSYLP GAGDESIELC
601RPLDSRLEHV DFKCLFKCLS VCHLIRVCAS LLLERRVIFV ANSLSTLSKC GHAVVATLYP
661FTWQHTYIPV LPASMIDIVC SPTPFLIGIL SCSLPQLQDL PIEEVLIVDL CADKFLQEVS
721DEDEILPPKL QAALMQILEE RNEILTQEQN FSQDVTLNSL VSEAFVRFFV ELVGHYSLNM
781TVTERGERVF QREPFRKSHT SRSVRHFLDL FMETQMFAGF IQDRELRKSG VKGLFEIRAI
841QYLETIPESE PSGMNRILRS LGSKMKFLQK K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

871 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.