Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DENN / MADD Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117157-100
Catalog Number: LS-G117157
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DENN(MADD) (NM_003682), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DENN | IG20 | KIAA0358 | Rab3 GDP/GTP exchange factor | MADD | RAB3GEP
- Origin Species
- Human
- NCBI GenBank
- NM_003682
- SwissProt
- Q8WXG6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 183.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVQKKKFCPR LLDYLVIVGA RHPSSDSVAQ TPELLRRYPL EDHTEFPLPP DVVFFCQPEG61CLSVRQRRMS LRDDTSFVFT LTDKDTGVTR YGICVNFYRS FQKRISKEKG EGGAGSRGKE121GTHATCASEE GGTESSESGS SLQPLSADST PDVNQSPRGK RRAKAGSRSR NSTLTSLCVL181SHYPFFSTFR ECLYTLKRLV DCCSERLLGK KLGIPRGVQR DTMWRIFTGS LLVEEKSSAL241LHDLREIEAW IYRLLRSPVP VSGQKRVDIE VLPQELQPAL TFALPDPSRF TLVDFPLHLP301LELLGVDACL QVLTCILLEH KVVLQSRDYN ALSMSVMAFV AMIYPLEYMF PVIPLLPTCM361ASAEQLLLAP TPYIIGVPAS FFLYKLDFKM PDDVWLVDLD SNRVIAPTNA EVLPILPEPE421SLELKKHLKQ ALASMSLNTQ PILNLEKFHE GQEIPLLLGR PSNDLQSTPS TEFNPLIYGN481DVDSVDVATR VAMVRFFNSA NVLQGFQMHT RTLRLFPRPV VAFQAGSFLA SRPRQTPFAE541KLARTQAVEY FGEWILNPTN YAFQRIHNNM FDPALIGDKP KWYAHQLQPI HYRVYDSNSQ601LAEALSVPPE RDSDSEPTDD SGSDSMDYDD SSSSYSSLGD FVSEMMKCDI NGDTPNVDPL661THAALGDASE VEIDELQNQK EAEEPGPDSE NSQENPPLRS SSSTTASSSP STVIHGANSE721PADSTEMDDK AAVGVSKPLP SVPPSIGKSN MDRRQAEIGE GSVRRRIYDN PYFEPQYGFP781PEEDEDEQGE SYTPRFSQHV SGNRAQKLLR PNSLRLASDS DAESDSRASS PNSTVSNTST841EGFGGIMSFA SSLYRNHSTS FSLSNLTLPT KGAREKATPF PSLKVFGLNT LMEIVTEAGP901GSGEGNRRAL VDQKSSVIKH SPTVKREPPS PQGRSSNSSE NQQFLKEVVH SVLDGQGVGW961LNMKKVRRLL ESEQLRVFVL SKLNRMVQSE DDARQDIIPD VEISRKVYKG MLDLLKCTVL1021SLEQSYAHAG LGGMASIFGL LEIAQTHYYS KEPDKRKRSP TESVNTPVGK DPGLAGRGDP1081KAMAQLRVPQ LGPRAPSATG KGPKELDTRS LKEENFIASI ELWNKHQEVK KQKALEKQRP1141EVIKPVFDLG ETEEKKSQIS ADSGVSLTSS SQRTDQDSVI GVSPAVMIRS SSQDSEVSTV1201VSNSSGETLG ADSDLSSNAG DGPGGEGSVH LASSRGTLSD SEIETNSATS TIFGKAHSLK1261PSIKEKLAGS PIRTSEDVSQ RVYLYEGLLG RDKGSMWDQL EDAAMETFSI SKERSTLWDQ1321MQFWEDAFLD AVMLEREGMG MDQGPQEMID RYLSLGEHDR KRLEDDEDRL LATLLHNLIS1381YMLLMKVNKN DIRKKVRRLM GKSHIGLVYS QQINEVLDQL ANLNGRDLSI WSSGSRHMKK1441QTFVVHAGTD TNGDIFFMEV CDDCVVLRSN IGTVYERWWY EKLINMTYCP KTKVLCLWRR1501NGSETQLNKF YTKKCRELYY CVKDSMERAA ARQQSIKPGP ELGGEFPVQD LKTGEGGLLQ1561VTLEGINLKF MHNQVFIELN HIKKCNTVRG VFVLEEFVPE IKEVVSHKYK TPMAHEICYS1621VLCLFSYVAA VHSSEEDLRT PPRPVSSSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1647 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.