LSBio
DDX49 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DDX49 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111464-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DDX49 (NM_019070), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DDX49 | DEAD box protein 49 | R27090_2
Origin Species
Human
NCBI GenBank
NM_019070
SwissProt
Q9Y6V7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MAGFAELGLS SWLVEQCRQL GLKQPTPVQL GCIPAILEGR DCLGCAKTGS GKTAAFVLPI
61LQKLSEDPYG IFCLVLTPTR ELAYQIAEQF RVLGKPLGLK DCIIVGGMDM VAQALELSRK
121PHVVIATPGR LADHLRSSNT FSIKKIRFLV MDEADRLLEQ GCTDFTVDLE AILAAVPARR
181QTLLFSATLT DTLRELQGLA TNQPFFWEAQ APVSTVEQLD QRYLLVPEKV KDAYLVHLIQ
241RFQDEHEDWS IIIFTNTCKT CQILCMMLRK FSFPTVALHS MMKQKERFAA LAKFKSSIYR
301ILIATDVASR GLDIPTVQVV INHNTPGLPK IYIHRVGRTA RAGRQGQAIT LVTQYDIHLV
361HAIEEQIKKK LEEFSVEEAE VLQILTQVNV VRRECEIKLE AAHFDEKKEI NKRKQLILEG
421KDPDLEAKRK AELAKIKQKN RRFKEKVEET LKRQKAGRAG HKGRPPRTPS GSHSGPVPSQ
481GLV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

483 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.