Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DDX33 / DHX33 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111308-100
Catalog Number: LS-G111308
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DHX33 (NM_020162), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DEAH box protein 33 | DHX33 | DDX33
- Origin Species
- Human
- NCBI GenBank
- NM_020162
- SwissProt
- Q9H6R0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPEEAGFPPA KRFRPGSGPP SRAGSFPPGR QVVMLLTAGS GGRGGGGGRR QQPPLAQPSA61SPYPEAVELQ RRSLPIFQAR GQLLAQLRNL DNAVLIGETG SGKTTQIPQY LYEGGISRQG121IIAVTQPRRV AAISLATRVS DEKRTELGKL VGYTVRFDDV TSEDTRIKFL TDGMLLREAI181SDSLLRKYSC VILDEAHERT IHTDVLFGVV KAAQKRRKEL GKLPLKVIVM SATMDVDLFS241QYFNGAPVLY LEGRQHPIQV FYTKQPQNDY LHAALVSVFQ IHQEAPSSQD ILVFLTGQEE301IEAMSKTCRD IAKHLPDGCP AMLVLPLYAS LPYAQQLRVF QGAPKGYRKV IISTNIAETS361ITITGIKYVV DTGMVKAKKY NPDSGLEVLA VQRVSKTQAW QRTGRAGRED SGICYRLYTE421DEFEKFDKMT VPEIQRCNLA SVMLQLLAMK VPNVLTFDFM SKPSPDHIQA AIAQLDLLGA481LEHKDDQLTL TPMGRKMAAF PLEPKFAKTI LMSPKFHCTE EILTIVSLLS VDSVLHNPPS541RREEVQGVRK KFISSEGDHM TLLNIYRTFK NLGGNKDWCK ENFVNSKNMT LVAEVRAQLR601DICLKMSMPI ASSRGDVESV RRCLAHSLFM STAELQPDGT YATTDTHQPV AIHPSSVLFH661CKPACVVYTE LLYTNKCYMR DLCVIDAQWL YEAAPEYFRR KLRTARNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
707 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.