LSBio
DDX28 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DDX28 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DDX28 antibodies →
$504.00each
Catalog Number: LS-G101558-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DDX28 (NM_018380), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DDX28 | MDDX28
Origin Species
Human
NCBI GenBank
NM_018380

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MALARPVRLF SLVTRLLLAP RRGLTVRSPD EPLPVVRIPV ALQRQLEQRQ SRRRNLPRPV
61LVRPGPLLVS ARRPELNQPA RLTLGRWERA PLASQGWKSR RARRDHFSIE RAQQEAPAVR
121KLSSKGSFAD LGLEPRVLHA LQEAAPEVVQ PTTVQSSTIP SLLRGRHVVC AAETGSGKTL
181SYLLPLLQRL LGQPSLDSLP IPAPRGLVLV PSRELAQQVR AVAQPLGRSL GLLVRDLEGG
241HGMRRIRLQL SRQPSADVLV ATPGALWKAL KSRLISLEQL SFLVLDEADT LLDESFLELV
301DYILEKSHIA EGPADLEDPF NPKAQLVLVG ATFPEGVGQL LNKVASPDAV TTITSSKLHC
361IMPHVKQTFL RLKGADKVAE LVHILKHRDR AERTGPSGTV LVFCNSSSTV NWLGYILDDH
421KIQHLRLQGQ MPALMRVGIF QSFQKSSRDI LLCTDIASRG LDSTGVELVV NYDFPPTLQD
481YIHRAGRVGR VGSEVPGTVI SFVTHPWDVS LVQKIELAAR RRRSLPGLAS SVKEPLPQAT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

540 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.