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DDX27 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DDX27 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112152-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DDX27 (NM_017895), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DEAD box protein 27 | DDX27 | DRS1 | HSPC259 | PP3241 | RNA helicase-like protein | RHLP | Rrp3p | DJ686N3.1
Origin Species
Human
NCBI GenBank
NM_017895
SwissProt
Q96GQ7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVLAQRRRGG CEKLRAGPQA VLASGSGFCD NMLADLGLIG TIGEDDEVPV EPESDSGDEE
61EEGPIVLGRR QKALGKNRSA DFNPDFVFTE KEGTYDGSWA LADVMSQLKK KRAATTLDEK
121IEKVRKKRKT EDKEAKSGKL EKEKEAKEGS EPKEQEDLQE NDEEGSEDEA SETDYSSADE
181NILTKADTLK VKDRKKKKKK GQEAGGFFED ASQYDENLSF QDMNLSRPLL KAITAMGFKQ
241PTPIQKACIP VGLLGKDICA CAATGTGKTA AFALPVLERL IYKPRQAPVT RVLVLVPTRE
301LGIQVHSVTR QLAQFCNITT CLAVGGLDVK SQEAALRAAP DILIATPGRL IDHLHNCPSF
361HLSSIEVLIL DEADRMLDEY FEEQMKEIIR MCSHHRQTML FSATMTDEVK DLASVSLKNP
421VRIFVNSNTD VAPFLRQEFI RIRPNREGDR EAIVAALLTR TFTDHVMLFT QTKKQAHRMH
481ILLGLMGLQV GELHGNLSQT QRLEALRRFK DEQIDILVAT DVAARGLDIE GVKTVINFTM
541PNTIKHYVHR VGRTARAGRA GRSVSLVGED ERKMLKEIVK AAKAPVKARI LPQDVILKFR
601DKIEKMEKDV YAVLQLEAEE KEMQQSEAQI NTAKRLLEKG KEAVVQEPER SWFQTKEERK
661KEKIAKALQE FDLALRGKKK RKKFMKDAKK KGEMTAEERS QFEILKAQMF AERLAKRNRR
721AKRARAMPEE EPVRGPAKKQ KQGKKSVFDE ELTNTSKKAL KQYRAGPSFE ERKQLGLPHQ
781RRGGNFKSKS RYKRRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

796 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.