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DDX19A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DDX19A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DDX19A antibodies →
$504.00each
Catalog Number: LS-G111804-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DDX19A (NM_018332), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DDX19-like protein | DDX19A | DDX19L | DEAD box protein 19A | DDX19-DDX19L | RNA helicase
Origin Species
Human
NCBI GenBank
NM_018332
SwissProt
Q9NUU7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MATDSWALAV DEQEAAVKSM TNLQIKEEKV KADTNGIIKT STTAEKTDEE EKEDRAAQSL
61LNKLIRSNLV DNTNQVEVLQ RDPNSPLYSV KSFEELRLKP QLLQGVYAMG FNRPSKIQEN
121ALPMMLAEPP QNLIAQSQSG TGKTAAFVLA MLSRVEPSDR YPQCLCLSPT YELALQTGKV
181IEQMGKFYPE LKLAYAVRGN KLERGQKISE QIVIGTPGTV LDWCSKLKFI DPKKIKVFVL
241DEADVMIATQ GHQDQSIRIQ RMLPRNCQML LFSATFEDSV WKFAQKVVPD PNVIKLKREE
301ETLDTIKQYY VLCSSRDEKF QALCNLYGAI TIAQAMIFCH TRKTASWLAA ELSKEGHQVA
361LLSGEMMVEQ RAAVIERFRE GKEKVLVTTN VCARGIDVEQ VSVVINFDLP VDKDGNPDNE
421TYLHRIGRTG RFGKRGLAVN MVDSKHSMNI LNRIQEHFNK KIERLDTDDL DEIEKIANTR
481TRPLEQKLIS EEDLAANDIL DYKDDDDKV

509 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.