Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DDO / D-Aspartate Oxidase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100090-100
Catalog Number: LS-G100090
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human D aspartate oxidase(DDO) (NM_003649), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DDO | DDO-1 | DDO-2 | D-aspartate oxidase | Aspartic oxidase | DASOX
- Origin Species
- Human
- NCBI GenBank
- NM_003649
- SwissProt
- Q99489
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRPARHWETR FGARDFGGFQ DCFFRDRLMD TARIAVVGAG VVGLSTAVCI SKLVPRCSVT61IISDKFTPDT TSDVAAGMLI PHTYPDTPIH TQKQWFRETF NHLFAIANSA EAGDAGVHLV121SGWQIFQSTP TEEVPFWADV VLGFRKMTEA ELKKFPQYVF GQAFTTLKCE CPAYLPWLEK181RIKGSGGWTL TRRIEDLWEL HPSFDIVVNC SGLGSRQLAG DSKIFPVRGQ VLQVQAPWVE241HFIRDGSGLT YIYPGTSHVT LGGTRQKGDW NLSPDAENSR EILSRCCALE PSLHGACNIR301EKVGLRPYRP GVRLQTELLA RDGQRLPVVH HYGHGSGGIS VHWGTALEAA RLVSECVHAL361RTPIPKSNLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
369 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.