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DDIAS Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DDIAS Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DDIAS antibodies →
$504.00each
Catalog Number: LS-G106793-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C11orf82(DDIAS) (NM_145018), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C11orf82 | Noxin
Origin Species
Human
NCBI GenBank
NM_145018
SwissProt
Q8IXT1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNRRRKFLLA SVLALQNSSF IYPSCQKCFS RIILVSKRSN CPKCGSTGES GNANYRYKLS
61LKVAESNKLF VITVFGSCLD TFFGLTATGL HRYIQDPNKI PETLDNDTTQ NLLTKAVETC
121FVGQSFIFGV TNFENQPGQG SDASNFLQQC SDHKRKAKAL VACQIVLPDP GIAGFTVIDY
181FHQLLQTFNF RKLQCDSQAP NNHLLALDHS NSDLSSTYTS DSTSDFFKSC SKDTFSKFWQ
241PSLEFTCIVS QLTDNDDFSA SEQSKAFGTL QQNRKSISIA EATGSSSCHD PIQDSWSLVS
301YMDKKSTAEK LGKELGLQAK ELSAVHSSHH EIGVNDSNLF SLEMREPLES SNTKSFHSAV
361EIKNRSQHEL PCFQHHGIDT PTSLQKRSAC CPPSLLRLEE TASSSQDGDP QIWDDLPFSE
421SLNKFLAVLE SEIAVTQADV SSRKHHVDND IDKFHADHSS LSVTPQRTTG ALHTPPIALR
481SSQVIVKANC SKDDFLFNCK GNLSPSVEKE SQPDNKVEAV SVNHNGRDMS EYFLPNPYLS
541ALSSSSKDLE TIVTLKKTIR ISPHRESDHS SLNNKYLNGC GEISVSEMNE KLTTLCYRKY
601NDVSDLCKLE NKQYCRWSKN QDDSFTICRK LTYPLETLCN SPNRSTNTLK EMPWGHINNN
661VTQSYSIGYE GSYDASADLF DDIAKEMDIA TEITKKSQDI LLKWGTSLAE SHPSESDFSL
721RSLSEDFIQP SQKLSLQSLS DSRHSRTCSP TPHFQSDSEY NFENSQDFVP CSQSTPISGF
781HQTRIHGINR AFKKPVFYSD LDGNYEKIRI FPENDKQQAS PSCPKNIKTP SQKIRSPIVS
841GISQPDVFNH YPFAECHETD SDEWVPPTTQ KIFPSDMLGF QGIGLGKCLA AYHFPDQQEL
901PRKKLKHIRQ GTNKGLIKKK LKNMLAAVVT KKKTHKYNCK SSGWISKCPD IQVLAAPQLH
961PILGPDSCSE VKCCLPFSEK GPPSVCETRS AWSPELFS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

998 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.