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DDHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DDHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109451-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DDHD1 (NM_030637), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DDHD domain containing 1 | DDHD1 | KIAA1705 | PA-PLA1 | Phospholipase DDHD1 | SPG28 | PAPLA1
Origin Species
Human
NCBI GenBank
NM_001160147
SwissProt
Q8NEL9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNYPGRGSPR SPEHNGRGGG GGAWELGSDA RPAFGGGVCC FEHLPGGDPD DGDVPLALLR
61GEPGLHLAPG TDDHNHHLAL DPCLSDENYD FSSAESGSSL RYYSEGESGG GGSSLSLHPP
121QQPPLVPTNS GGGGATGGSP GERKRTRLGG PAARHRYEVV TELGPEEVRW FYKEDKKTWK
181PFIGYDSLRI ELAFRTLLQT TGARPQGGDR DGDHVCSPTG PASSSGEDDD EDRACGFCQS
241TTGHEPEMVE LVNIEPVCVR GGLYEVDVTQ GECYPVYWNQ ADKIPVMRGQ WFIDGTWQPL
301EEEESNLIEQ EHLNCFRGQQ MQENFDIEVS KSIDGKDAVH SFKLSRNHVD WHSVDEVYLY
361SDATTSKIAR TVTQKLGFSK ASSSGTRLHR GYVEEATLED KPSQTTHIVF VVHGIGQKMD
421QGRIIKNTAM MREAARKIEE RHFSNHATHV EFLPVEWRSK LTLDGDTVDS ITPDKVRGLR
481DMLNSSAMDI MYYTSPLYRD ELVKGLQQEL NRLYSLFCSR NPDFEEKGGK VSIVSHSLGC
541VITYDIMTGW NPVRLYEQLL QKEEELPDER WMSYEERHLL DELYITKRRL KEIEERLHGL
601KASSMTQTPA LKFKVENFFC MGSPLAVFLA LRGIRPGNTG SQDHILPREI CNRLLNIFHP
661TDPVAYRLEP LILKRYSNIS PVQIHWYNTS NPLPYEHMKP SFLNPAKEPT SVSENEGIST
721IPSPVTSPVL SRRHYGESIT NIGKASILGA ASIGKGLGGM LFSRFGRSST TQSSETSKDS
781MEDEKKPVAS PSATTVGTQT LPHSSSGFLD SALELDHRID FELREGLVES RYWSAVTSHT
841AYWSSLDVAL FLLTFMYKHE HDDDAKPNLD PI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

872 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.