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DCST2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DCST2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DCST2 antibodies →
$725.00each
Catalog Number: LS-G106966-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DCST2 (NM_144622), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPKVMKDVVH PLGGEEPSMA RAVVRSVGGF TLGLSLATAY GLLELLVEGH SPWGCLVGTL
61TLAAFLSLGM GFSRQVRATV LLLLPQAFSR QGRTLLLVAA FGLVLQGPCA NTLRNFTRAS
121EAVACGAELA LNQTAEVLQR AKQPLVSALN KIKAIARKTK EVADRVRKFF RSIMDGVKHI
181ARALRNVWQW LLHIGDVCNS ELGNPYLKCA RVFDDAKDSC MMVIPQAYHL CYVLMPFKLA
241LCGLASLVQV FCVIPKYIQP FLRQTIGTPV IQLLNRVRQE FEFNMTATHH FSVDLNASRS
301LSQVAMDLHE AVSMKLHRVR EALALMGFTT PLLLVLLYLQ ALFYRYCYLN WDHYDNIYIT
361SRFLRMEAVR STAGLPTVLP LSAHEARRYI PPGSIFLSQW EKFFYILETF NLIRHLLLVL
421FLVFLDYAVF WVLDLARHQL QGEIVARSPV LVSLTVEGTG YAGNIYRDLV SAFDVLQQGN
481ISILSRRCLL RPSEPDSTGY IVIGVMYGLC FFITLFGSYV SRLRRVICAS YYPSREQERI
541SYLYNVLLSR RTNLLAALHR SVRRRAADQG HRSAFLVLAS RCPCLGPFVS HFWLHQAYCL
601GCGQPQDEGD MENTVSCSTP GCQGLYCLTC FRLLDNTCSV CASPLSYQGD LDLELDSSDE
661EGPQLWLAAA QRKDPEQAWL LQQQLQEVLG RSLSMESTSE SSDLDEEKGP QQRKHGQQPL
721PEAHQPVSIL TSPEPHRPPE TSSATKGAPT PASEPSVPLS PPSLPDPSHP PPK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

773 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.