LSBio
DCDC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DCDC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DCDC5 antibodies →
$725.00each
Catalog Number: LS-G131172-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DCDC5 (NM_020869), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_020869

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWPVLPSGQL NEEFDWPIQG LLVPSSPPMK KPICKTTEPY APVRLRVLQN GEKNKNRSVT
61ILGPDISPGR KTQCTEILNL PSAARRLYNE KGKEIFALKD LQRDELVYVS CGELWINPDL
121SIAQQKKQIF LRNLESDIAK IQIFCSTHKI EALVLEVQSD IVSGSKLAVH KPVAIFGEEK
181QVTEPEEKQM QEDPLTTENA SSEILDSHVR AHLRMKACHT LPRYAWQETS HDFDEDDSLP
241KKTEKGLFEN VEPQKKHSCS PKHSKLHKHC HQQFEYRDGQ IISHAAPQLV LGVQGPNLRS
301GMEVVLVEKK SDGSHQRWIH QEDSRTFHLV SNPDLVLAVS MTKTRNEVCG YPVIVQKYKP
361YNNGAANQKW HYMKNIKALV AFHSTALDKE ITSANYAGVC TSSVIKEENI DQPGYCYLSP
421DGKRKTMLCL ACGQSMRTEK GLKQLLPGVP FLCISGTKTQ KPFLQGPFKV ISVAEVDLSC
481DKAEKTLSYY QARLLSLRMK TCTQAASHSG MAATHQKAVK IIAYKNGDGY RNGKLIVAGT
541FPMLLTECTE QLGLARAASK VYTKDGTPIF TLRDLVLWAL DESFLQRDSE KQKQDAAPVG
601KEQIIVEKNP RMKVKNRLFA KSVTSDSLDG IDKSLLTLIL RNPIAIWVSC GEPFLPPNAL
661QKAEKLEKQN WLKKDRILAD LDTMRHKMRQ LKGRRVAACQ PATMVPTKSP VQPVVVEGGW
721TEQTQQEIKL MELIRHTEAH LSEIQEMESK INFPIATKRI AVKPSNLYKQ PNTKRVWIYL
781NGGRPEDGTY AWGKTISELL QDCSSRLKMT HPARALYTPS GEPIQSWDDI ERDMVICVSM
841GHGFKTPKEL KQLMEIRANY ARIRRQQGPQ ATDIVVSPST KLLSLAHLHN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

890 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.