Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DCC1 / DSCC1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110055-100
Catalog Number: LS-G110055
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DSCC1 (NM_024094), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DCC1 | DSCC1 | HDCC1
- Origin Species
- Human
- NCBI GenBank
- NM_024094
- SwissProt
- Q9BVC3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKRTRDEVDA TLQIAKLNAA ELLPAVHCLG FGPGASGAAA GDFCLLELEP TLCQQLEDGH61SLVIRGDKDE QAVLCSKDKT YDLKIADTSN MLLFIPGCKT PDQLKKEDSH CNIIHTEIFG121FSNNYWELRR RRPKLKKLKK LLMENPYEGP DSQKEKDSNS SKYTTEDLLD QIQASEEEIM181TQLQVLNACK IGGYWRILEF DYEMKLLNHV TQLVDSESWS FGKVPLNTCL QELGPLEPEE241MIEHCLKCYG KKYVDEGEVY FELDADKICR AAARMLLQNA VKFNLAEFQE VWQQSVPEGM301VTSLDQLKGL ALVDRHSRPE IIFLLKVDDL PEDNQERFNS LFSLREKWTE EDIAPYIQDL361CGEKQTIGAL LTKYSRSSMQ NGVKVYNSRR PISTRTRPLE QKLISEEDLA ANDILDYKDD421DDKV
424 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.