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DCC Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DCC Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DCC antibodies →
$725.00each
Catalog Number: LS-G116102-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DCC (NM_005215), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CRC18 | Colorectal tumor suppressor | DCC | Colorectal cancer suppressor | IGDCC1 | Netrin receptor DCC | Tumor suppressor protein DCC | CRCR1
Origin Species
Human
NCBI GenBank
NM_005215
SwissProt
P43146

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
158.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENSLRCVWV PKLAFVLFGA SLLSAHLQVT GFQIKAFTAL RFLSEPSDAV TMRGGNVLLD
61CSAESDRGVP VIKWKKDGIH LALGMDERKQ QLSNGSLLIQ NILHSRHHKP DEGLYQCEAS
121LGDSGSIISR TAKVAVAGPL RFLSQTESVT AFMGDTVLLK CEVIGEPMPT IHWQKNQQDL
181TPIPGDSRVV VLPSGALQIS RLQPGDIGIY RCSARNPASS RTGNEAEVRI LSDPGLHRQL
241YFLQRPSNVV AIEGKDAVLE CCVSGYPPPS FTWLRGEEVI QLRSKKYSLL GGSNLLISNV
301TDDDSGMYTC VVTYKNENIS ASAELTVLVP PWFLNHPSNL YAYESMDIEF ECTVSGKPVP
361TVNWMKNGDV VIPSDYFQIV GGSNLRILGV VKSDEGFYQC VAENEAGNAQ TSAQLIVPKP
421AIPSSSVLPS APRDVVPVLV SSRFVRLSWR PPAEAKGNIQ TFTVFFSREG DNRERALNTT
481QPGSLQLTVG NLKPEAMYTF RVVAYNEWGP GESSQPIKVA TQPELQVPGP VENLQAVSTS
541PTSILITWEP PAYANGPVQG YRLFCTEVST GKEQNIEVDG LSYKLEGLKK FTEYSLRFLA
601YNRYGPGVST DDITVVTLSD VPSAPPQNVS LEVVNSRSIK VSWLPPPSGT QNGFITGYKI
661RHRKTTRRGE METLEPNNLW YLFTGLEKGS QYSFQVSAMT VNGTGPPSNW YTAETPENDL
721DESQVPDQPS SLHVRPQTNC IIMSWTPPLN PNIVVRGYII GYGVGSPYAE TVRVDSKQRY
781YSIERLESSS HYVISLKAFN NAGEGVPLYE SATTRSITDP TDPVDYYPLL DDFPTSVPDL
841STPMLPPVGV QAVALTHDAV RVSWADNSVP KNQKTSEVRL YTVRWRTSFS ASAKYKSEDT
901TSLSYTATGL KPNTMYEFSV MVTKNRRSST WSMTAHATTY EAAPTSAPKD LTVITREGKP
961RAVIVSWQPP LEANGKITAY ILFYTLDKNI PIDDWIMETI SGDRLTHQIM DLNLDTMYYF
1021RIQARNSKGV GPLSDPILFR TLKVEHPDKM ANDQGRHGDG GYWPVDTNLI DRSTLNEPPI
1081GQMHPPHGSV TPQKNSNLLV IIVVTVGVIT VLVVVIVAVI CTRRSSAQQR KKRATHSAGK
1141RKGSQKDLRP PDLWIHHEEM EMKNIEKPSG TDPAGRDSPI QSCQDLTPVS HSQSETQLGS
1201KSTSHSGQDT EEAGSSMSTL ERSLAARRAP RAKLMIPMDA QSNNPAVVSA IPVPTLESAQ
1261YPGILPSPTC GYPHPQFTLR PVPFPTLSVD RGFGAGRSQS VSEGPTTQQP PMLPPSQPEH
1321SSSEEAPSRT IPTACVRPTH PLRSFANPLL PPPMSAIEPK VPYTPLLSQP GPTLPKTHVK
1381TASLGLAGKA RSPLLPVSVP TAPEVSEESH KPTEDSANVY EQDDLSEQMA SLEGLMKQLN
1441AITGSAF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1447 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.