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DCBLD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DCBLD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DCBLD2 antibodies →
$725.00each
Catalog Number: LS-G102207-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DCBLD2 (NM_080927), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DCBLD2 | CLCP1 | ESDN
Origin Species
Human
SwissProt
Q96PD2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MASRAVVRAR RCPQCPQVRA AAAAPAWAAL PLSRSLPPCS NSSSFSMPLF LLLLLVLLLL
61LEDAGAQQGD GCGHTVLGPE SGTLTSINYP QTYPNSTVCE WEIRVKMGER VRIKFGDFDI
121EDSDSCHFNY LRIYNGIGVS RTEIGKYCGL GLQMNHSIES KGNEITLLFM SGIHVSGRGF
181LASYSVIDKQ DLITCLDTAS NFLEPEFSKY CPAGCLLPFA EISGTIPHGY RDSSPLCMAG
241VHAGVVSNTL GGQISVVISK GIPYYESSLA NNVTSVVGHL STSLFTFKTS GCYGTLGMES
301GVIADPQITA SSVLEWTDHT GQENSWKPKK ARLKKPGPPW AAFATDEYQW LQIDLNKEKK
361ITGIITTGST MVEHNYYVSA YRILYSDDGQ KWTVYREPGV EQDKIFQGNK DYHQDVRNNF
421LPPIIARFIR VNPTQWQQKI AMKMELLGCQ FIPKGRPPKL TQPPPPRNSN DLKNTTAPPK
481IAKGRAPKFT QPLQPRSSNE FPAQTEQTTA SPDIRNTTVT PNVTKDVALA AVLVPVLVMV
541LTTLILILVC AWHWRNRKKK TEGTYDLPYW DRAGWWKGMK QFLPAKAVDH EETPVRYSSS
601EVNHLSPREV TTVLQADSAE YAQPLVGGIV GTLHQRSTFK PEEGKEAGYA DLDPYNSPGQ
661EVYHAYAEPL PITGPEYATP IIMDMSGHPT TSVGQPSTST FKATGNQPPP LVGTYNTLLS
721RTDSCSSAQA QYDTPKAGKP GLPAPDELVY QVPQSTQEVS GAGRDGECDV FKEIL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

775 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.