LSBio
DCAF8L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DCAF8L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DCAF8L1 antibodies →
$725.00each
Catalog Number: LS-G121294-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DCAF8L1 (NM_001017930), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001017930

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSHQEGSTGG LPDLVTESLF SSPEEQSGVA AVTAASSDIE MAATEPSTGD GGDTRDGGFL
61NDASTENQNT DSESSSEDVE LESMGEGLFG YPLVGEETER EEEEEEMEEE GEEEEQPRMC
121PRCGGTNHDQ CLLDEDQALE EWISSETSAL PRSRWQVLTA LRQRQLGSSA RFVYEACGAR
181TFVQRFRLQY LLGSHAGSVS TIHFNQRGTR LASSGDDLRV IVWDWVRQKP VLNFESGHDI
241NVIQAKFFPN CGDSTLAMCG HDGQVRVAEL INASYCENTK RVAKHRGPAH ELALEPDSPY
301KFLTSGEDAV VFTIDLRQDR PASKVVVTRE NDKKVGLYTI SMNPANIYQF AVGGHDQFVR
361IYDQRRIDKK ENNGVLKKFT PHHLVYCDFP TNITCVVYSH DGTELLASYN DEDIYLFNSS
421LSDGAQYVKR YKGHRNNDTI KCVNFYGPRS EFVVSGSDCG HVFFWEKSSS QIIQFMEGDR
481GDIVNCLEPH PYLPVLATSG LDQHVRIWTP TAKTATELTG LKDVIKKNKQ ERDEDNLNYT
541DSFDNRMLRF FVRHLLQRAH QPGWRDHGAE FPDEEELDES SSTSDTSEEE GQDRVQCIPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

600 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.