Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DCAF6 / NRIP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111702-100
Catalog Number: LS-G111702
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NRIP(DCAF6) (NM_018442), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARCAP | PC326 | IQ motif and WD repeats 1 | RP4-745I14.1 | DCAF6 | IQWD1 | MSTP055 | NRIP
- Origin Species
- Human
- NCBI GenBank
- NM_018442
- SwissProt
- Q58WW2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRGGSYPHL LWDVRKRSLG LEDPSRLRSR YLGRREFIQR LKLEATLNVH DGCVNTICWN61DTGEYILSGS DDTKLVISNP YSRKVLTTIR SGHRANIFSA KFLPCTNDKQ IVSCSGDGVI121FYTNVEQDAE TNRQCQFTCH YGTTYEIMTV PNDPYTFLSC GEDGTVRWFD TRIKTSCTKE181DCKDDILINC RRAATSVAIC PPIPYYLAVG CSDSSVRIYD RRMLGTRATG NYAGRGTTGM241VARFIPSHLN NKSCRVTSLC YSEDGQEILV SYSSDYIYLF DPKDDTAREL KTPSAEERRE301ELRQPPVKRL RLRGDWSDTG PRARPESERE RDGEQSPNVS LMQRMSDMLS RWFEEASEVA361QSNRGRGRSR PRGGTSQSDI STLPTVPSSP DLEVSETAME VDTPAEQFLQ PSTSSTMSAQ421AHSTSSPTES PHSTPLLSSP DSEQRQSVEA SGHHTHHQSD SPSSVVNKQL GSMSLDEQQD481NNNEKLSPKP GTGEPVLSLH YSTEGTTTST IKLNFTDEWS SIASSSRGIG SHCKSEGQEE541SFVPQSSVQP PEGDSETKAP EESSEDVTKY QEGVSAENPV ENHINITQSD KFTAKPLDSN601SGERNDLNLD RSCGVPEESA SSEKAKEPET SDQTSTESAT NENNTNPEPQ FQTEATGPSA661HEETSTRDSA LQDTDDSDDD PVLIPGARYR AGPGDRRSAV ARIQEFFRRR KERKEMEELD721TLNIRRPLVK MVYKGHRNSR TMIKEANFWG ANFVMSGSDC GHIFIWDRHT AEHLMLLEAD781NHVVNCLQPH PFDPILASSG IDYDIKIWSP LEESRIFNRK LADEVITRNE LMLEETRNTI841TVPASFMLRM LASLNHIRAD RLEGDRSEGS GQENENEDEETRTRPLEQKLISEEDLAANDILDYKDDDDKV
880 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.