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DCAF15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DCAF15 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DCAF15 antibodies →
$504.00each
Catalog Number: LS-G107335-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DCAF15 (NM_138353), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_138353

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPSSKSERN SGAGSGGGGP GGAGGKRAAG RRREHVLKQL ERVKISGQLS PRLFRKLPPR
61VCVSLKNIVD EDFLYAGHIF LGFSKCGRYV LSYTSSSGDD DFSFYIYHLY WWEFNVHSKL
121KLVRQVRLFQ DEEIYSDLYL TVCEWPSDAS KVIVFGFNTR SANGMLMNMM MMSDENHRDI
181YVSTVAVPPP GRCAACQDAS RAHPGDPNAQ CLRHGFMLHT KYQVVYPFPT FQPAFQLKKD
241QVVLLNTSYS LVACAVSVHS AGDRSFCQIL YDHSTCPLAP ASPPEPQSPE LPPALPSFCP
301EAAPARSSGS PEPSPAIAKA KEFVADIFRR AKEAKGGVPE EARPALCPGP SGSRCRAHSE
361PLALCGETAP RDSPPASEAP ASEPGYVNYT KLYYVLESGE GTEPEDELED DKISLPFVVT
421DLRGRNLRPM RERTAVQGQY LTVEQLTLDF EYVINEVIRH DATWGHQFCS FSDYDIVILE
481VCPETNQVLI NIGLLLLAFP SPTEEGQLRP KTYHTSLKVA WDLNTGIFET VSVGDLTEVK
541GQTSGSVWSS YRKSCVDMVM KWLVPESSGR YVNRMTNEAL HKGCSLKVLA DSERYTWIVL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

600 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.