Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DCAF12 / WDR40A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113237-100
Catalog Number: LS-G113237
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DCAF12 (NM_015397), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CT102 | Cancer/testis antigen 102 | DCAF12 | DKFZP434O125 | KIAA1892 | TCC52 | WDR40A | WD repeat domain 40A
- Origin Species
- Human
- NCBI GenBank
- AK000354
- SwissProt
- Q5T6F0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MARKVVSRKR KAPASPGAGS DAQGPQFGWD HSLHKRKRLP PVKRSLVYYL KNREVRLQNE61TSYSRVLHGY AAQQLPSLLK EREFHLGTLN KVFASQWLNH RQVVCGTKCN TLFVVDVQTS121QITKIPILKD QEPGGVTQQG CGIHAIELNP SRTLLATGGD NPNSLAIYRL PTLDPVCVGD181DGHKDWIFSI AWISDTMAVS GSRDGSMGLW EVTDDVLTKS DARHNVSRVP VYAHITHKAL241KDIPKEDTNP DNCKVRALAF NNKNKELGAV SLDGYFHLWK AENTLSKLLS TKLPYCRENV301CLAYGSEWSV YAVGSQAHVS FLDPRQPSYN VKSVCSRERG SGIRSVSFYE HIITVGTGQG361SLLFYDIRAQ RFLEERLSAC YGSKPRLAGE NLKLTTGKGW LNHDETWRNY FSDIDFFPNA421VYTHCYDSSG TKLFVAGGPL PSGLHGNYAG LWSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
453 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.