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DBF4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DBF4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G100892-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DBF4 (NM_006716), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Activator of S phase kinase | CHIF | DBF4 homolog (S. cerevisiae) | Chiffon homolog A | DBF4 | DBF4A | ZDBF1 | Protein DBF4 homolog A | ASK
Origin Species
Human
NCBI GenBank
NM_006716
SwissProt
Q9UBU7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSGAMRIHS KGHFQGGIQV KNEKNRPSLK SLKTDNRPEK SKCKPLWGKV FYLDLPSVTI
61SEKLQKDIKD LGGRVEEFLS KDISYLISNK KEAKFAQTLG RISPVPSPES AYTAETTSPH
121PSHDGSSFKS PDTVCLSRGK LLVEKAIKDH DFIPSNSILS NALSWGVKIL HIDDIRYYIE
181QKKKELYLLK KSSTSVRDGG KRVGSGAQKT RTGRLKKPFV KVEDMSQLYR PFYLQLTNMP
241FINYSIQKPC SPFDVDKPSS MQKQTQVKLR IQTDGDKYGG TSIQLQLKEK KKKGYCECCL
301QKYEDLETHL LSEQHRNFAQ SNQYQVVDDI VSKLVFDFVE YEKDTPKKKR IKYSVGSLSP
361VSASVLKKTE QKEKVELQHI SQKDCQEDDT TVKEQNFLYK ETQETEKKLL FISEPIPHPS
421NELRGLNEKM SNKCSMLSTA EDDIRQNFTQ LPLHKNKQEC ILDISEHTLS ENDLEELRVD
481HYKCNIQASV HVSDFSTDNS GSQPKQKSDT VLFPAKDLKE KDLHSIFTHD SGLITINSSQ
541EHLTVQAKAP FHTPPEEPNE CDFKNMDSLP SGKIHRKVKI ILGRNRKENL EPNAEFDKRT
601EFITQEENRI CSSPVQSLLD LFQTSEEKSE FLGFTSYTEK SGICNVLDIW EEENSDNLLT
661AFFSSPSTST FTGF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

674 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.