LSBio
DAXX Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DAXX Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DAXX antibodies →
$725.00each
Catalog Number: LS-G125825-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DAXX (NM_001141970), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BING2 | CENP-C binding protein | DAXX | DAP6 | Death-associated protein 6 | EAP1 | Fas-binding protein | HDaxx | ETS1-associated protein 1
Origin Species
Human
NCBI GenBank
NM_001350
SwissProt
Q9UER7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRGSENCGEG RLERRFLSII VLDDDDEDEA AAQPGPSHPL PNAASPGAEA PSSSEPHGAR
61GSSSSGGKKC YKLENEKLFE EFLELCKMQT ADHPEVVPFL YNRQQRAHSL FLASAEFCNI
121LSRVLSRARS RPAKLYVYIN ELCTVLKAHS AKKKLNLAPA ATTSNEPSGN NPPTHLSLDP
181TNAENTASQS PRTRGSRRQI QRLEQLLALY VAEIRRLQEK ELDLSELDDP DSAYLQEARL
241KRKLIRLFGR LCELKDCSSL TGRVIEQRIP YRGTRYPEVN RRIERLINKP GPDTFPDYGD
301VLRAVEKAAA RHSLGLPRQQ LQLMAQDAFR DVGIRLQERR HLDLIYNFGC HLTDDYRPGV
361DPALSDPVLA RRLRENRSLA MSRLDEVISK YAMLQDKSEE GERKKRRARL QGTSSHSADT
421PEASLDSGEG PSGMASQGCP SASRAETDDE DDEESDEEEE EEEEEEEEEA TDSEEEEDLE
481QMQEGQEDDE EEDEEEEAAA GKDGDKSPMS SLQISNEKNL EPGKQISRSS GEQQNKGRIV
541SPSLLSEEPL APSSIDAESN GEQPEELTLE EESPVSQLFE LEIEALPLDT PSSVETDISS
601SRKQSEEPFT TVLENGAGMV SSTSFNGGVS PHNWGDSGPP CKKSRKEKKQ TGSGPLGNSY
661VERQRSVHEK NGKKICTLPS PPSPLASLAP VADSSTRVDS PSHGLVTSSL CIPSPARLSQ
721TPHSQPPRPG TCKTSVATQC DPEEIIVLSD SD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

752 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.