Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DAW1 / WDR69 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104587-100
Catalog Number: LS-G104587
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR69(DAW1) (NM_178821), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_178821
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLKSLLLRY YPPGIMLEYE KHGELKTKSI DLLDLGPSTD VSALVEEIQK AEPLLTASRT61EQVKLLIQRL QEKLGQNSNH TFYLFKVLKA HILPLTNVAL NKSGSCFITG SYDRTCKLWD121TASGEELNTL EGHRNVVYAI AFNNPYGDKI ATGSFDKTCK LWSVETGKCY HTFRGHTAEI181VCLSFNPQST LVATGSMDTT AKLWDIQNGE EVYTLRGHSA EIISLSFNTS GDRIITGSFD241HTVVVWDADT GRKVNILIGH CAEISSASFN WDCSLILTGS MDKTCKLWDA TNGKCVATLT301GHDDEILDSC FDYTGKLIAT ASADGTARIF SAATRKCIAK LEGHEGEISK ISFNPQGNHL361LTGSSDKTAR IWDAQTGQCL QVLEGHTDEI FSCAFNYKGN IVITGSKDNT CRIWRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
415 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.