LSBio
DACH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DACH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DACH2 antibodies →
$504.00each
Catalog Number: LS-G125813-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DACH2 (NM_001139514), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DACH2 | Dachshund homolog 2
Origin Species
Human
NCBI GenBank
NM_053281
SwissProt
Q96NX9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVSASPVIS ATSSGAGVPG GLFRAEPLYS TPREPPRLTP NMINSFVVNN HSNSAGGGGR
61GNTNTNECRM VDMHGMKVAS FLMDGQELIC LPQVFDLFLK HLVGGLHTVY TKLKRLDISP
121VVCTVEQVRI LRGLGAIQPG VNRCKLITRK DFETLFTDCT NASSRPGRPP KRSLGVLQEN
181ARLLTHAVPG LLSPGLITPT GITAAAMAEA MKLQKMKLMA MNTLQGNGSQ NGTESEPDDL
241NSNTGGSESS WDKDKMQSPF AAPGPQHGIA HAALAGQPGI GGAPTLNPLQ QNHLLTNRLD
301LPFMMMPHPL LPVSLPPASV AMAMNQMNHL NTIANMAAAA QIHSPLSRAG TSVIKERIPE
361SPSPAPSLEE NHRPGSQTSS HTSSSVSSSP SQMDHHLERM EEVPVQIPIM KSPLDKIQLT
421PGQALPAGFP GPFIFADSLS SVETLLTNIQ GLLKVALDNA RIQEKQIQQE KKELRLELYR
481EREIRENLER QLAVELQSRT TMQKRLKKEK KTKRKLQEAL EFESKRREQV EQALKQATTS
541DSGLRMLKDT GIPDIEIENN GTPHDSAAMQ A
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

571 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.