DACH / DACH1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107777-100
Catalog Number: LS-G107777
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DACH1 (NM_080759), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DACH | Dachshund homolog (Drosophila) | Dachshund homolog 1 | DACH1
- Origin Species
- Human
- NCBI GenBank
- NM_004392
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAVPAALIPP TQLVPPQPPI STSASSSGTT TSTSSATSSP APSIGPPASS GPTLFRPEPI61ASAAAAAATV TSTGGGGGGG GSGGGGGSSG NGGGGGGGGG GSNCNPNLAA ASNGSGGGGG121GISAGGGVAS STPINASTGS SSSSSSSSSS SSSSSSSSSS SSSCGPLPGK PVYSTPSPVE181NTPQNNECKM VDLRGAKVAS FTVEGCELIC LPQAFDLFLK HLVGGLHTVY TKLKRLEITP241VVCNVEQVRI LRGLGAIQPG VNRCKLISRK DFETLYNDCT NASSRPGRPP KRTQSVTSPE301NSHIMPHSVP GLMSPGIIPP TGLTAAAAAA AAATNAAIAE AMKVKKIKLE AMSNYHASNN361QHGADSENGD MNSSVGLELP FMMMPHPLIP VSLPPASVTM AMSQMNHLST IANMAAAAQV421QSPPSRVETS VIKERVPDSP SPAPSLEEGR RPGSHPSSHR SSSVSSSPAR TESSSDRIPV481HQNGLSMNQM LMGLSPNVLP GPKEGDLAGH DMGHESKRMH IEKDETPLST PTARDSLDKL541SLTGHGQPLP PGFPSPFLFP DGLSSIETLL TNIQGLLKVA IDNARAQEKQ VQLEKTELKM601DFLRERELRE TLEKQLAMEQ KNRAIVQKRL KKEKKAKRKL QEALEFETKR REQAEQTLKQ661AASTDSLRVL NDSLTPEIEA DRSGGRTDAE RTIQDGRLYL KTTVMYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
706 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.