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DAB2IP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DAB2IP Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108735-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DAB2IP (NM_032552), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AIP1 | AF9Q34 | ASK-interacting protein 1 | ASK1-interacting protein 1 | DAB2 interaction protein | DAB2-interacting protein | DOC-2/DAB2 interactive protein | DAB2 interacting protein | DIP1/2 | KIAA1743 | NGAP-like protein | DAB2IP
Origin Species
Human
SwissProt
Q5VWQ8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPDSLLDQD DSYESPQERP GSRRSLPGSL SEKSPSMEPS AATPFRVTGF LSRRLKGSIK
61RTKSQPKLDR NHSFRHILPG FRSAAAAAAD NERSHLMPRL KESRSHESLL SPSSAVEALD
121LSMEEEVVIK PVHSSILGQD YCFEVTTSSG SKCFSCRSAA ERDKWMENLR RAVHPNKDNS
181RRVEHILKLW VIEAKDLPAK KKYLCELCLD DVLYARTTGK LKTDNVFWGE HFEFHNLPPL
241RTVTVHLYRE TDKKKKKERN SYLGLVSLPA ASVAGRQFVE KWYPVVTPNP KGGKGPGPMI
301RIKARYQTIT ILPMEMYKEF AEHITNHYLG LCAALEPILS AKTKEEMASA LVHILQSTGK
361VKDFLTDLMM SEVDRCGDNE HLIFRENTLA TKAIEEYLKL VGQKYLQDAL GEFIKALYES
421DENCEVDPSK CSAADLPEHQ GNLKMCCELA FCKIINSYCV FPRELKEVFA SWRQECSSRG
481RPDISERLIS ASLFLRFLCP AIMSPSLFNL LQEYPDDRTA RTLTLIAKVT QNLANFAKFG
541SKEEYMSFMN QFLEHEWTNM QRFLLEISNP ETLSNTAGFE GYIDLGRELS SLHSLLWEAV
601SQLEQSIVSK LGPLPRILRD VHTALSTPGS GQLPGTNDLA STPGSGSSSI SAGLQKMVIE
661NDLSGLIDFT RLPSPTPENK DLFFVTRSSG VQPSPARSSS YSEANEPDLQ MANGGKSLSM
721VDLQDARTLD GEAGSPAGPD VLPTDGQAAA AQLVAGWPAR ATPVNLAGLA TVRRAGQTPT
781TPGTSEGAPG RPQLLAPLSF QNPVYQMAAG LPLSPRGLGD SGSEGHSSLS SHSNSEELAA
841AAKLGSFSTA AEELARRPGE LARRQMSLTE KGGQPTVPRQ NSAGPQRRID QPPPPPPPPP
901PAPRGRTPPN LLSTLQYPRP SSGTLASASP DWVGPSTRLR QQSSSSKGDS PELKPRAVHK
961QGPSPVSPNA LDRTAAWLLT MNAQLLEDEG LGPDPPHRDR LRSKDELSQA EKDLAVLQDK
1021LRISTKKLEE YETLFKCQEE TTQKLVLEYQ ARLEEGEERL RRQQEDKDIQ MKGIISRLMS
1081VEEELKKDHA EMQAAVDSKQ KIIDAQEKRI ASLDAANARL MSALTQLKES MH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1132 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.