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DAB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DAB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DAB1 antibodies →
$725.00each
Catalog Number: LS-G101713-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DAB1 (NM_021080), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DAB1 | Disabled homolog 1
Origin Species
Human
NCBI GenBank
NM_021080
SwissProt
O75553

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSTETELQVA VKTSAKKDSR KKGQDRSEAT LIKRFKGEGV RYKAKLIGID EVSAARGDKL
61CQDSMMKLKG VVAGARSKGE HKQKIFLTIS FGGIKIFDEK TGALQHHHAV HEISYIAKDI
121TDHRAFGYVC GKEGNHRFVA IKTAQAAEPV ILDLRDLFQL IYELKQREEL EKKAQKDKQC
181EQAVYQTILE EDVEDPVYQY IVFEAGHEPI RDPETEENIY QVPTSQKKEG VYDVPKSQPV
241SAVTQLELFG DMSTPPDITS PPTPATPGDA FIPSSSQTLP ASADVFSSVP FGTAAVPSGY
301VAMGAVLPSF WGQQPLVQQQ MVMGAQPPVA QVMPGAQPIA WGQPGLFPAT QQPWPTVAGQ
361FPPAAFMPTQ TVMPLPAAMF QGPLTPLATV PGTSDSTRSS PQTDKPRQKM GKETFKDFQM
421AQPPPVPSRK PDQPSLTCTS EAFSSYFNKV GVAQDTDDCD DFDISQLNLT PVTSTTPSTN
481SPPTPAPRQS SPSKSSASHA SDPTTDDIFE EGFESPSKSE EQEAPDGSQA SSNSDPFGEP
541SGEPSGDNIS PQAGS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

555 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.