Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CYTH4 / PSCD4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114272-100
Catalog Number: LS-G114272
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PSCD4(CYTH4) (NM_013385), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CYTH4 | Cytohesin-4 | CYT4 | Cytohesin 4 | DJ63G5.1 | PSCD4
- Origin Species
- Human
- NCBI GenBank
- NM_013385
- SwissProt
- Q9UIA0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDLCHPEPAE LSSGETEELQ RIKWHRKQLL EDIQKLKDEI ADVFAQIDCF ESAEESRMAQ61KEKELCIGRK KFNMDPAKGI QYFIEHKLLT PDVQDIARFL YKGEGLNKTA IGTYLGERDP121INLQVLQAFV DCHEFANLNL VQALRQFLWS FRLPGEAQKI DRMMEAFATR YCLCNPGVFQ181STDTCYVLSF SIIMLNTSLH NPNVRDRPPF ERFVSMNRGI NNGSDLPEDQ LRNLFDSIKS241EPFSIPEDDG NDLTHTFFNP DREGWLLKLG GRVKTWKRRW FILTDNCLYY FEFTTDKEPR301GIIPLENLSV QKVDDPKKPF CLELYNPSCR GQKIKACKTD GDGRVVEGKH ESYRISATSA361EERDQWIESI RASITRVPFY DLVSTRKKKI ASKQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
394 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.