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CYP4X1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CYP4X1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CYP4X1 antibodies →
$504.00each
Catalog Number: LS-G104768-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CYP4X1 (NM_178033), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CYPIVX1 | Cytochrome P450 4X1 | CYP4X1
Origin Species
Human
SwissProt
Q8N118

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEFSWLETRW ARPFYLAFVF CLALGLLQAI KLYLRRQRLL RDLRPFPAPP THWFLGHQKF
61IQDDNMEKLE EIIEKYPRAF PFWIGPFQAF FCIYDPDYAK TLLSRTDPKS QYLQKFSPPL
121LGKGLAALDG PKWFQHRRLL TPGFHFNILK AYIEVMAHSV KMMLDKWEKI CSTQDTSVEV
181YEHINSMSLD IIMKCAFSKE TNCQTNSTHD PYAKAIFELS KIIFHRLYSL LYHSDIIFKL
241SPQGYRFQKL SRVLNQYTDT IIQERKKSLQ AGVKQDNTPK RKYQDFLDIV LSAKDESGSS
301FSDIDVHSEV STFLLAGHDT LAASISWILY CLALNPEHQE RCREEVRGIL GDGSSITWDQ
361LGEMSYTTMC IKETCRLIPA VPSISRDLSK PLTFPDGCTL PAGITVVLSI WGLHHNPAVW
421KNPKVFDPLR FSQENSDQRH PYAYLPFSAG SRNCIGQEFA MIELKVTIAL ILLHFRVTPD
481PTRPLTFPNH FILKPKNGMY LHLKKLSEC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

509 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.