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CYP4F8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CYP4F8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114755-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CYPIVF8(CYP4F8) (NM_007253), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CPF8 | Cytochrome P450 4F8 | CYP4F8 | CYPIVF8
Origin Species
Human
NCBI GenBank
NM_007253
SwissProt
P98187

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLLSLSWLG LRPVAASPWL LLLVVGASWL LARILAWTYA FYHNGRRLRC FPQPRKQNWF
61LGHLGLVTPT EEGLRVLTQL VATYPQGFVR WLGPITPIIN LCHPDIVRSV INTSDAITDK
121DIVFYKTLKP WLGDGLLLSV GDKWRHHRRL LTPAFHFNIL KPYIKIFSKS ANIMHAKWQR
181LAMEGSTCLD VFEHISLMTL DSLQKCIFSF DSNCQEKPSE YITAIMELSA LVVKRNNQFF
241RYKDFLYFLT PCGRRFHRAC RLVHDFTDAV IQERRRTLTS QGVDDFLQAK AKSKTLDFID
301VLLLSEDKNG KELSDEDIRA EADTFMFGGH DTTASGLSWV LYNLARHPEY QERCRQEVQE
361LLKDREPKEI EWDDLAQLPF LTMCLKESLR LHPPIPTFAR GCTQDVVLPD SRVIPKGNVC
421NINIFAIHHN PSVWPDPEVY DPFRFDPENA QKRSPMAFIP FSAGPRNCIG QKFAMAEMKV
481VLALTLLRFR ILPDHREPRR TPEIVLRAED GLWLRVEPLG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

520 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.