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CYP4F11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CYP4F11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CYP4F11 antibodies →
$504.00each
Catalog Number: LS-G110713-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CYPIVF11(CYP4F11) (NM_021187), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CYP4F11 | CYPIVF11 | Cytochrome P450 4F11
Origin Species
Human
NCBI GenBank
NM_021187
SwissProt
Q9HBI6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60 kda
Protein Type
Lysate
Amino Acid Sequence
1MPQLSLSWLG LGPVAASPWL LLLLVGGSWL LARVLAWTYT FYDNCRRLQC FPQPPKQNWF
61WGHQGLVTPT EEGMKTLTQL VTTYPQGFKL WLGPTFPLLI LCHPDIIRPI TSASAAVAPK
121DMIFYGFLKP WLGDGLLLSG GDKWSRHRRM LTPAFHFNIL KPYMKIFNKS VNIMHDKWQR
181LASEGSARLD MFEHISLMTL DSLQKCVFSF ESNCQEKPSE YIAAILELSA FVEKRNQQIL
241LHTDFLYYLT PDGQRFRRAC HLVHDFTDAV IQERRRTLPT QGIDDFLKNK AKSKTLDFID
301VLLLSKDEDG KELSDEDIRA EADTFMFEGH DTTASGLSWV LYHLAKHPEY QEQCRQEVQE
361LLKDREPIEI EWDDLAQLPF LTMCIKESLR LHPPVPVISR CCTQDFVLPD GRVIPKGIVC
421LINIIGIHYN PTVWPDPEVY DPFRFNQENI KERSPLAFIP FSAGPRNCIG QAFAMAEMKV
481VLALTLLHFR ILPTHTEPRR KPELILRAEG GLWLRVEPLG ANSQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

524 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.