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CYP4B1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CYP4B1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CYP4B1 antibodies →
$504.00each
Catalog Number: LS-G122992-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CYP4B1 (NM_000779), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cytochrome P450 4B1 | CYPIVB1 | CYP4B1 | Cytochrome p450 IVb1 | Cytochrome P450-HP | P-450HP
Origin Species
Human
NCBI GenBank
NM_000779
SwissProt
P13584

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVPSFLSLSF SSLGLWASGL ILVLGFLKLI HLLLRRQTLA KAMDKFPGPP THWLFGHALE
61IQETGSLDKV VSWAHQFPYA HPLWFGQFIG FLNIYEPDYA KAVYSRGDPK APDVYDFFLQ
121WIGRGLLVLE GPKWLQHRKL LTPGFHYDVL KPYVAVFTES TRIMLDKWEE KAREGKSFDI
181FCDVGHMALN TLMKCTFGRG DTGLGHSRDS SYYLAVSDLT LLMQQRLVSF QYHNDFIYWL
241TPHGRRFLRA CQVAHDHTDQ VIRERKAALQ DEKVRKKIQN RRHLDFLDIL LGARDEDDIK
301LSDADLRAEV DTFMFEGHDT TTSGISWFLY CMALYPEHQH RCREEVREIL GDQDFFQWDD
361LGKMTYLTMC IKESFRLYPP VPQVYRQLSK PVTFVDGRSL PAGSLISMHI YALHRNSAVW
421PDPEVFDSLR FSTENASKRH PFAFMPFSAG PRNCIGQQFA MSEMKVVTAM CLLRFEFSLD
481PSRLPIKMPQ LVLRSKNGFH LHLKPLGPGS GK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

512 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.