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CYP3A43 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CYP3A43 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CYP3A43 antibodies →
$725.00each
Catalog Number: LS-G107961-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CYP3A43 (NM_057095), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cytochrome P450 3A43 | CYP3A43 | Cytochrome P450 polypeptide 43
Origin Species
Human
NCBI GenBank
NM_022820
SwissProt
Q9HB55

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLIPNFAME TWVLVATSLV LLYIYGTHSH KLFKKLGIPG PTPLPFLGTI LFYLRGLWNF
61DRECNEKYGE MWGLYEGQQP MLVIMDPDMI KTVLVKECYS VFTNQMPLGP MGFLKSALSF
121AEDEEWKRIR TLLSPAFTSV KFKEMVPIIS QCGDMLVRSL RQEAENSKSI NLKDFFGAYT
181MDVITGTLFG VNLDSLNNPQ DPFLKNMKKL LKLDFLDPFL LLISLFPFLT PVFEALNIGL
241FPKDVTHFLK NSIERMKESR LKDKQKHRVD FFQQMIDSQN SKETKSHKAL SDLELVAQSI
301IIIFAAYDTT STTLPFIMYE LATHPDVQQK LQEEIDAVLP NKAPVTYDAL VQMEYLDMVV
361NETLRLFPVV SRVTRVCKKD IEINGVFIPK GLAVMVPIYA LHHDPKYWTE PEKFCPERFS
421KKNKDSIDLY RYIPFGAGPR NCIGMRFALT NIKLAVIRAL QNFSFKPCKE TQIPLKLDNL
481PILQPEKPIV LKVHLRDGIT SGP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

503 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.