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CYP2W1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CYP2W1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CYP2W1 antibodies →
$504.00each
Catalog Number: LS-G112229-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CYP2W1 (NM_017781), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CYP2W1 | CYPIIW1 | Cytochrome P450 2W1
Origin Species
Human
NCBI GenBank
NM_017781
SwissProt
Q8TAV3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MALLLLLFLG LLGLWGLLCA CAQDPSPAAR WPPGPRPLPL VGNLHLLRLS QQDRSLMELS
61ERYGPVFTVH LGRQKTVVLT GFEAVKEALA GPGQELADRP PIAIFQLIQR GGGIFFSSGA
121RWRAARQFTV RALHSLGVGR EPVADKILQE LKCLSGQLDG YRGRPFPLAL LGWAPSNITF
181ALLFGRRFDY RDPVFVSLLG LIDEVMVLLG SPGLQLFNVY PWLGALLQLH RPVLRKIEEV
241RAILRTLLEA RRPHVCPGDP VCSYVDALIQ QGQGDDPEGL FAEANAVACT LDMVMAGTET
301TSATLQWAAL LMGRHPDVQG RVQEELDRVL GPGRTPRLED QQALPYTSAV LHEVQRFITL
361LPHVPRCTAA DTQLGGFLLP KGTPVIPLLT SVLLDETQWQ TPGQFNPGHF LDANGHFVKR
421EAFLPFSAGR RVCVGERLAR TELFLLFAGL LQRYRLLPPP GVSPASLDTT PARAFTMRPR
481AQALCAVPRP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

490 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.