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CYP2S1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CYP2S1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CYP2S1 antibodies →
$504.00each
Catalog Number: LS-G109487-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CYP2S1 (NM_030622), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CYP2S1 | CYPIIS1 | Cytochrome P450 2S1
Origin Species
Human
NCBI GenBank
NM_030622
SwissProt
Q96SQ9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MEATGTWALL LALALLLLLT LALSGTRARG HLPPGPTPLP LLGNLLQLRP GALYSGLMRL
61SKKYGPVFTI YLGPWRPVVV LVGQEAVREA LGGQAEEFSG RGTVAMLEGT FDGHGVFFSN
121GERWRQLRKF TMLALRDLGM GKREGEELIQ AEARCLVETF QGTEGRPFDP SLLLAQATSN
181VVCSLLFGLR FSYEDKEFQA VVRAAGGTLL GVSSQGGQTY EMFSWFLRPL PGPHKQLLHH
241VSTLAAFTVR QVQQHQGNLD ASGPARDLVD AFLLKMAQEE QNPGTEFTNK NMLMTVIYLL
301FAGTMTVSTT VGYTLLLLMK YPHVQKWVRE ELNRELGAGQ APSLGDRTRL PYTDAVLHEA
361QRLLALVPMG IPRTLMRTTR FRGYTLPQGT EVFPLLGSIL HDPNIFKHPE EFNPDRFLDA
421DGRFRKHEAF LPFSLGKRVC LGEGLAKAEL FLFFTTILQA FSLESPCPPD TLSLKPTVSG
481LFNIPPAFQL QVRPTDLHST TQTR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

504 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.