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CYP2J2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CYP2J2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CYP2J2 antibodies →
$504.00each
Catalog Number: LS-G122996-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cytochrome p450 2J2(CYP2J2) (NM_000775), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Arachidonic acid epoxygenase | CYP2J2 | CYPIIJ2 | Cytochrome p450 IIj2 | CPJ2 | Cytochrome P450 2J2
Origin Species
Human
NCBI GenBank
NM_000775
SwissProt
P51589

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MLAAMGSLAA ALWAVVHPRT LLLGTVAFLL AADFLKRRRP KNYPPGPWRL PFLGNFFLVD
61FEQSHLEVQL FVKKYGNLFS LELGDISAVL ITGLPLIKEA LIHMDQNFGN RPVTPMREHI
121FKKNGLIMSS GQAWKEQRRF TLTALRNFGL GKKSLEERIQ EEAQHLTEAI KEENGQPFDP
181HFKINNAVSN IICSITFGER FEYQDSWFQQ LLKLLDEVTY LEASKTCQLY NVFPWIMKFL
241PGPHQTLFSN WKKLKLFVSH MIDKHRKDWN PAETRDFIDA YLKEMSKHTG NPTSSFHEEN
301LICSTLDLFF AGTETTSTTL RWALLYMALY PEIQEKVQAE IDRVIGQGQQ PSTAARESMP
361YTNAVIHEVQ RMGNIIPLNV PREVTVDTTL AGYHLPKGTM ILTNLTALHR DPTEWATPDT
421FNPDHFLENG QFKKREAFMP FSIGKRACLG EQLARTELFI FFTSLMQKFT FRPPNNEKLS
481LKFRMGITIS PVSHRLCAVP QV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

502 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.