Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CYP2C18 / CYP2C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122998-100
Catalog Number: LS-G122998
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CYP2C18(CYP2C18) (NM_000772), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CYP2C17 | CYP2C18 | CYPIIC18 | Cytochrome P450 2C18 | Cytochrome P450-6b/29c | p450IIC17 | p450-6B/29C | CPCI | CYP2C | Unspecific monooxygenase
- Origin Species
- Human
- NCBI GenBank
- NM_000772
- SwissProt
- P33260
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPAVALVLC LSCLFLLSLW RQSSGRGRLP SGPTPLPIIG NILQLDVKDM SKSLTNFSKV61YGPVFTVYFG LKPIVVLHGY EAVKEALIDH GEEFSGRGSF PVAEKVNKGL GILFSNGKRW121KEIRRFCLMT LRNFGMGKRS IEDRVQEEAR CLVEELRKTN ASPCDPTFIL GCAPCNVICS181VIFHDRFDYK DQRFLNLMEK FNENLRILSS PWIQVCNNFP ALIDYLPGSH NKIAENFAYI241KSYVLERIKE HQESLDMNSA RDFIDCFLIK MEQEKHNQQS EFTVESLIAT VTDMFGAGTE301TTSTTLRYGL LLLLKYPEVT AKVQEEIECV VGRNRSPCMQ DRSHMPYTDA VVHEIQRYID361LLPTNLPHAV TCDVKFKNYL IPKGTTIITS LTSVLHNDKE FPNPEMFDPG HFLDKSGNFK421KSDYFMPFSA GKRMCMGEGL ARMELFLFLT TILQNFNLKS QVDPKDIDIT PIANAFGRVP481PLYQLCFIPVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
490 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.