Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CYP-M / CYP20A1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102480-100
Catalog Number: LS-G102480
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CYP20A1 (NM_177538), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CYP20A1 | CYP-M | Cytochrome P450 20A1 | Cytochrome P450 monooxygenase
- Origin Species
- Human
- NCBI GenBank
- NM_177538
- SwissProt
- Q6UW02
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLDFAIFAVT FLLALVGAVL YLYPASRQAA GIPGITPTEE KDGNLPDIVN SGSLHEFLVN61LHERYGPVVS FWFGRRLVVS LGTVDVLKQH INPNKTLDPF ETMLKSLLRY QSGGGSVSEN121HMRKKLYENG VTDSLKSNFA LLLKLSEELL DKWLSYPETQ HVPLSQHMLG FAMKSVTQMV181MGSTFEDDQE VIRFQKNHGT VWSEIGKGFL DGSLDKNMTR KKQYEDALMQ LESVLRNIIK241ERKGRNFSQH IFIDSLVQGN LNDQQILEDS MIFSLASCII TAKLCTWAIC FLTTSEEVQK301KLYEEINQVF GNGPVTPEKI EQLRYCQHVL CETVRTAKLT PVSAQFQDIE GKIDRFIIPR361ETLVLYALGV VLQDPNTWPS PHKFDPDRFD DELVMKTFSS LGFSGTQECP ELRFAYMVTT421VLLSVLVKRL HLLSVEGQVI ETKYELVTSS REEAWITVSK RYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
462 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.