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CYLC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CYLC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CYLC1 antibodies →
$725.00each
Catalog Number: LS-G110766-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cylicin 1(CYLC1) (NM_021118), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CYL1 | Cylicin I | Cylicin-1 | CYL | Cylicin 1 | CYCL1 | CYLC1 | Multiple-band polypeptide I
Origin Species
Human
NCBI GenBank
Z22780
SwissProt
P35663

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLPRLLKVN IRTYDNSIPI SESSRKSWNQ KHFALTFPKP LQRGTNDKSR PLKSQITVTR
61HDKRKLEEGQ KPAHKWIRHS FRKILQWPPI YTAAREQTPF RHLYTSKTHL KKAEYKKSKD
121EKGGTPLKKD SKKKGGSYAT NPESKQIVEE KTKRQNEADK TPLKSSHENE QSKKSKSSSE
181TNPESQNSKT VSKNCSQKDK KDSKNSKKTN TEFLHTKNNP KKDLKRSKTS NDPISEICSE
241NSLNVDFLML VGQSDDESIN FDAWLRNYSQ NNSKNYSLKY TKYTKKDTKK NAKKSSDAES
301EDSKDAKKDS KKVKKNVKKD DKKKDVKKDT ESTDAESGDS KDERKDTKKD KKKLKKDDKK
361KDTKKYPEST DTESGDAKDA RNDSRNLKKA SKNDDKKKDA KKITFSTDSE SELESKESQK
421DEKKDKKDSK TDNKKSVKND EESTDADSEP KGDSKKGKKD EKKGKKDSKK DDKKKDAKKN
481AESTEMESDL ELKKDKKHSK EKKGSKKDIK KDARKDTEST DAEFDESSKT GFKTSTKIKG
541SDTESEESLY KPGAKKKIDE SDGTSANSKM EGLESKRGFR MSSKKTTFNE KGEKASTGRV
601PPSREKPPLP ACEPSLPSPK VRRLCWCKMP PPPPKPRYAP LPEAPWIHKL L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

651 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.