Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CXXC1 / CGBP Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101235-100
Catalog Number: LS-G101235
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CGBP(CXXC1) (NM_014593), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CXXC1 | CpG-binding protein | CXXC finger protein 1 | HCGBP | HsT2645 | PCCX1 | PHF18 | ZCGPC1 | CFP1 | CGBP | CpG binding protein | CXXC finger 1 (PHD domain)
- Origin Species
- Human
- NCBI GenBank
- NM_014593
- SwissProt
- Q9P0U4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGDGSDPEP PDAGEDSKSE NGENAPIYCI CRKPDINCFM IGCDNCNEWF HGDCIRITEK61MAKAIREWYC RECREKDPKL EIRYRHKKSR ERDGNERDSS EPRDEGGGRK RPVPDPDLQR121RAGSGTGVGA MLARGSASPH KSSPQPLVAT PSQHHQQQQQ QIKRSARMCG ECEACRRTED181CGHCDFCRDM KKFGGPNKIR QKCRLRQCQL RARESYKYFP SSLSPVTPSE SLPRPRRPLP241TQQQPQPSQK LGRIREDEGA VASSTVKEPP EATATPEPLS DEDLPLDPDL YQDFCAGAFD301DHGLPWMSDT EESPFLDPAL RKRAVKVKHV KRREKKSEKK KEERYKRHRQ KQKHKDKWKH361PERADAKDPA SLPQCLGPGC VRPAQPSSKY CSDDCGMKLA ANRIYEILPQ RIQQWQQSPC421IAEEHGKKLL ERIRREQQSA RTRLQEMERR FHELEAIILR AKQQAVREDE ESNEGDSDDT481DLQIFCVSCG HPINPRVALR HMERCYAKYE SQTSFGSMYP TRIEGATRLF CDVYNPQSKT541YCKRLQVLCP EHSRDPKVPA DEVCGCPLVR DVFELTGDFC RLPKRQCNRH YCWEKLRRAE601VDLERVRVWY KLDELFEQER NVRTAMTNRA GLLALMLHQT IQHDPLTTDL RSSADRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
656 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.