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CXorf59 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CXorf59 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CHDC2 antibodies →
$504.00each
Catalog Number: LS-G105299-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CHDC2 (NM_173695), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CHDC2 | CXorf59 | RP13-11B7.1
Origin Species
Human
NCBI GenBank
NM_173695
SwissProt
Q8N9S7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MAIHLDKQNI ILKNDKDEYL KKTRDGVLPP YQDAKPPSPA SIKKTYTTSK FNDAEPAKGN
61LFIGVEVLPE NLHLDESETS EEDHGSLEKE KYEQFLSLEE GTKAHYFFEK VVNAAQTWFS
121LFGWPEGPHS FSIPETIRRD VYKMQFYSST SPPQKFSRQN DFSKYNKTIY DVLLHLSGKM
181PPGINSSQSL PVDNHEKRVI QLHLQHSSLL DFLNAQGGCI SHVLPEFLLE PEDYKRWIEI
241MSSTNTMPVS SCTPKKKCSI VIEMSKFEAW SKRAWTDVFL QIYKVLVLSR VVPYCSNNMP
301PICVQNTPKV NPCFASSNIY SDSERILLSW MNINYENTRH VIWKNCHKDV IPSERWIVNF
361DKDLSDGLVF ATQLGAYCPF LIESHFINMY TRPKSPEEYL HNCLIIVNTL YEIDFDVEIQ
421VCLRVFPKEM DAGVSGLRKE DMPSVWVVTI RLAVAVARTK RQKKGDIQFA CFFVCLFFFI
481FVVISFSLWS RMHFFLLLPL DI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

502 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.